LightMix® BCR ABL for chronic myeloid leukemia and acute lymphoblastic leukemia

Kit for relative quantification of bcr-abl t(9;22) fusion transcript versus Abl1 (cDNA); detection of exon a2 of Abl1 and optional discrimination of common bcr-abl breakpoints using the Roche Diagnostics LightCycler® 1.x / 2.0 / 480 / 480 II, cobas z 480 Analyzer or Nano (MyGo). Lyophilized mix of primers and probes for a total of 96 reactions with a final volume of 20 μl each. These reagents were developed and manufactured by TIB MOLBIOL GmbH. For research-use (RUO) only.

Kit content

1 Vial containing premixed forward and reverse primers and probes for 96 reactions Abl1 transcript
1 Vial containing premixed probes and reverse (but no forward) primer for 96 reactions bcr-abl t(9;22) fusion transcript (reagents common to all translocation variants) and the Abl1 exon a2/a3 discrimination probe
1 Vial with exon1 forwar primer for bcr-abl t(9;22)
1 Vial with exon 19 forwar primer for bcr-abl t(9;22)
1 Vial with exon 13 forwar primer for bcr-abl t(9;22)
1 Standard row with 6 lyophilized plasmid standards 10^1 to 10^6 target equivalents per rxn 1 Sealing foil for the standard row

Additional requirements

LightCycler® FastStart DNA Master HybProbe (Cat.-No. 03003248001)
Transcriptor First Strand cDNA Synthesis Kit (Cat.-No.04896866001)
LightCycler® Capillaries, 20 μl (Cat.-No. 04929292001)
LightCycler® 480 Multiwell Plate 96, white (Cat.-No. 04729692001)