LightMix® Hemochromatosis type 1 (HFE) H63D S65C C282Y
Kit with reagents for the detection of common mutations in the HFE gene (OMIM: 235200) in genomic human DNA from nucleic acid extracts obtained from peripheral blood using the Roche Diagnostics LightCycler® 1.x / 2.0 / 480 / 480 II / Nano or cobas z 480 Analyzer. Lyophilized mix of primers and probes for a total of 96 reactions with a final volume of 20 μl each.These reagents were developed and manufactured by TIB MOLBIOL GmbH.
Kit Content
3 Vial containing premixed forward and reverse primers and probes for 32 reactions
1 Vial with Positive Control HFE Mutant 282Y; 32 reactions
1 Vial with Positive Control HFE Mutant 63D; 32 reactions
1 Vial with Positive Control HFE Mutant 65C; 32 reactions
Additional Requirements
LightCycler® FastStart DNA Master HybProbe (Cat.-No. 03003248001)
High Pure PCR Template Preparation Kit (Cat.-No. 11796828001) or
MagNA Pure 96 DNA and Viral NA Small Volume Kit (Cat.-No. 05467497001) or
MagNA Pure Compact Nucleic Acid Isolation Kit I (Cat.-No. 03730964001)
MagNA Pure LC DNA Isolation Kit I (Cat.-No. 03003990001)