LightMix® Polyomavirus JC BK

Kit with reagents for the detection of Polyomavirus JC and BK DNA using the Roche Diagnostics LightCycler® 1.x / 2.0 and 480 II (not LC480 1. version) Instruments or cobas z 480 analyzer. Lyophilized mix of primers and probes for a total of 96 reactions with a final volume of 20 μl each. These reagents were developed and manufactured by TIB MOLBIOL GmbH.

Kit content

3 Vials Parameter Specific Reagents (PSR) and Control Reaction containing premixed and lyophilized primers and hybridization probes for 32 reactions each 1 Standard row with 6 lyophilized cloned standard of JC from 10^1 to 10^6 target equivalents per rxn 1 Vials containing plasmid 1E3 copies/reaction: Positive Control JC 1 Standard row with 6 lyophilized cloned standard of BK from 10^1 to 10^6 target equivalents per rxn 1 Vials containingplasmid 1E5 copies/reaction: Positive Control BK 2 Vial containing Extraction Control Target (ECT): 4.8x 10^6 copies (total)
1 Vial containing the stabilizer for preparation of the 'No Template Control' (NTC)
2 Sealing foils to close the standard rows

Additional requirements

LightMix® Kit Color Compensation 530/640/690 (TIB MOLBIOL, Cat.-No. 05997704001)
LightCycler® FastStart DNA Master HybProbe (Cat.-No. 03003248001)
LightCycler® Capillaries, 20 μl (Cat.-No. 04929292001)
LightCycler® 480 Multiwell Plate 384, white (Cat.-No. 04729749001) or
LightCycler® 480 Multiwell Plate 96, white (Cat.-No. 04729692001)