LightMix® (Cytochrome P450) CYP 2D6 *3*4*5/5
Kit with reagents for the detection of the CYP 2D6 *3, *4 and *5/*5 polymorphisms using the Roche Diagnostics LightCycler® 1.x / 2.0 or 480 II. Lyophilized mix of primers and probes for a total of 96 reactions with a final volume of 20 μl each. These reagents were developed and manufactured by TIB MOLBIOL GmbH. For research-use (RUO) only.
Kit content
3 Vials containing lyophilized primers and probes for each 32 PCR reactions CYP 2D6
1 Vial with wt control DNA, 10^5 target equivalents per rxn 1 Vial with variant control DNA (*3 and *4), 10^5 target equivalents per rxn 1 Vial with mixed control DNA (*1/*3 and *1/*4), 10^5 target equivalents per rxn
Additional requirements
LightMix Color Compensation HybProbe (540/640/670/690) (Cat.-No. 05997704001)
LightCycler® FastStart DNA Master HybProbe (Cat.-No. 03003248001)
High Pure PCR Template Preparation Kit (Cat.-No. 11796828001)
LightCycler® Capillaries, 20 μl (Cat.-No. 04929292001)
LightCycler® 480 Multiwell Plate 384, white (Cat.-No. 04729749001) or
LightCycler® 480 Multiwell Plate 96, white (Cat.-No. 04729692001)