LightMix® Factor II Prothrombin
Kit with reagents for the detection of the Factor II (F2, prothrombin, OMIM 176930) G20210A mutation (NCBI dbSNP: rs1799963) from genomic human DNA extracted from peripheral blood using the Roche Diagnostics LightCycler® 1.x / 2.0 / 96 / 480 / 480 II / Nano or cobas z 480 Analyzer. Lyophilized mix of primers and probes for a total of 64 reactions with a final volume of 10 μl each.These reagents were developed and manufactured by TIB MOLBIOL GmbH.
Kit Content
1 Vial containing premixed forward and reverse primers and probes for 64 reactions
1 Vial with Positive Heterozygous Control (HT); 40 reactions
1 Vial with Genotyping Standard Wildtype (WT); 40 reactions
1 Vial with Genotyping Standard Mutant (MT); 40 reactions
Additional Requirements
LightCycler® FastStart DNA Master HybProbe (Cat.-No. 03003248001)
High Pure PCR Template Preparation Kit (Cat.-No. 11796828001) or
MagNA Pure 96 DNA and Viral NA Small Volume Kit (Cat.-No. 05467497001) or
MagNA Pure Compact Nucleic Acid Isolation Kit I (Cat.-No. 03730964001)
MagNA Pure LC DNA Isolation Kit I (Cat.-No. 03003990001)