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LightMix® Factor V Leiden

Art.

06896529001

Kit with reagents for the detection of the Factor V Leiden' mutation, the 1691G-A transition (NCBI dbSNP: rs6025) in exon 10 of the Coagulation Factor V gene (OMIM: 612309), resulting in amino acid substitution Arginin position 506 to Glutamin (R506Q or HGVS: NP_000121.2:p.Arg534Gln) from human genomic DNA extracted from peripheral blood using the Roche Diagnostics LightCycler® 1.x / 2.0 / 96 / 480 / 480 II / Nano or cobas z 480 Analyzer. Lyophilized mix of primers and probes for a total of 64 reactions with a final volume of 10 μl each.These reagents were developed and manufactured by TIB MOLBIOL GmbH.

Kit Content

1 Vial containing premixed forward and reverse primers and probes for 64 reactions
1 Vial with Positive Heterozygous Control (HT); 40 reactions
1 Vial with Genotyping Standard Wildtype (WT); 40 reactions
1 Vial with Genotyping Standard Mutant (MT); 40 reactions

Additional Requirements

LightCycler® FastStart DNA Master HybProbe (Cat.-No. 03003248001)
High Pure PCR Template Preparation Kit (Cat.-No. 11796828001) or
MagNA Pure 96 DNA and Viral NA Small Volume Kit (Cat.-No. 05467497001) or
MagNA Pure Compact Nucleic Acid Isolation Kit I (Cat.-No. 03730964001)
MagNA Pure LC DNA Isolation Kit I (Cat.-No. 03003990001)