LightMix Kit Norovirus

Art.

05945259001

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Kit with reagents for the 1-step RT-PCR detection of Norovirus GGI and GGII RNA and for the detection of Norovirus GGI and GGII cDNAusing the Roche Diagnostics LightCycler® 2.0 and 480 II Instruments. Lyophilized mix of primers and probes for a total of 96 reactions with a final volume of 20 μl each. These reagents were developed and manufactured by TIB MOLBIOL GmbH.

Kit Content

1 Vial containing premixed lyophilized primers and probes for 96 PCR reactions 1 Row with 6 lyophilized cloned plasmid DNA standards from 10^1 to 10^6 target equivalents per reaction of Norovirus GGII
1 Vial containing control DNA Norovirus GGI, 5 x 10^3 target equiv. per reaction 1 Vial containing control DNA Norovirus GGII , 5 x 10^3 target equiv. per reaction 1 Vial containing IC DNA MS2 phage, 5 x 10^3 target equivalents per reaction 1 Sealing foil for the standard row

Additional Requirements

LightCycler® FastStart DNA Master HybProbe (Cat.-No. 03003248001)
LightCycler® 480 RNA Master Hydrolysis Probes (Cat.-No. 04991885 001)
LightCycler® 480 RTR RNA Virus Master (Cat.-No. 05619416001)
LightCycler® Multicolor Demo Set (Cat.-No. 0362485400)
High Pure Viral Nucleic Acid Kit (Cat.-No.11858874001)
RNA, MS2 (Cat.-No. 10165948001)
LightCycler® Capillaries, 20 μl (Cat.-No. 04929292001)
LightCycler® 480 Multiwell Plate 384, white (Cat.-No. 04729749001) or
LightCycler® 480 Multiwell Plate 96, white (Cat.-No. 04729692001)