LightMix® Clostridium difficile

Art.

05945313001

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Kit with reagents for the detection of Clostridium difficile and identification of the tcdC deletion variant using the Roche Diagnostics LightCycler® 1.x / 2.0 / 480 II Instruments or cobas z 480 Analyzer. Lyophilized mix of primers and probes for a total of 96 reactions with a final volume of 20 μl each. These reagents were developed and manufactured by TIB MOLBIOL GmbH. For research-use (RUO) only.

Kit Content

3 Vials containing premixed primers and probes for 32 PCR reactions
3 Vials containing premixed primers and probes for 32 control reactions
1 Vial containing Extraction Control Target (ECT): 4.8x 10^6 copies (total)
1 Vial containing the stabilizer for preparation of the 'No Template Control' (NTC)
1 Standard row with 6 lyophilized standards of C.difficile del. 10^1 to 10^6 target equivalents per rxn 1 Sealing foil for the standard row
1 Vial containing control DNA C. difficile 10^5 target equivalents per rxn
1 Vial containing control DNA C. difficile del. 10^5 target equivalents per rxn

Additional Requirements

LightMix Color Compensation HybProbe (540/640/670/690) (Cat.-No. 05997704001)
LightCycler® FastStart DNA Master HybProbe (Cat.-No. 03003248001)
LightCycler® Capillaries, 20 μl (Cat.-No. 04929292001)
LightCycler® 480 Multiwell Plate 384, white (Cat.-No. 04729749001) or
LightCycler® 480 Multiwell Plate 96, white (Cat.-No. 04729692001)